Citations

TwistMethNext

Das, J. (2024). TwistMethNext (v1.0.0). Zenodo. https://doi.org/10.5281/zenodo.14204261

Pipeline tools

FastQC

Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].

Trim Galore

Felix Krueger, Frankie James, Phil Ewels, Ebrahim Afyounian, Michael Weinstein, Benjamin Schuster-Boeckler, Gert Hulselmans, & sclamons. (2023). FelixKrueger/TrimGalore: v0.6.10 - add default decompression path (0.6.10). Zenodo. https://doi.org/10.5281/zenodo.7598955

Bismark

Krueger, F., & Davies, R. (2019). Bismark: a versatile tool for aligning sequencing data to bisulfite-converted genomes. Bioinformatics, 35(4), 623-625. https://doi.org/10.1093/bioinformatics/bty724

SAMtools

Danecek P, Bonfield JK, Liddle J, et al. Twelve years of SAMtools and BCFtools. GigaScience. 2021;10(2):giab008. doi:10.1093/gigascience/giab008

QualiMap

Konstantin Okonechnikov, Ana Conesa, Fernando García-Alcalde, Qualimap 2: advanced multi-sample quality control for high-throughput sequencing data, Bioinformatics, Volume 32, Issue 2, January 2016, Pages 292–294, https://doi.org/10.1093/bioinformatics/btv566

MultiQC

Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.

EdgeR

Chen Y, Chen L, Lun ATL, Baldoni P, Smyth GK (2025). “edgeR v4: powerful differential analysis of sequencing data with expanded functionality and improved support for small counts and larger datasets.” Nucleic Acids Research, 53(2), gkaf018. doi:10.1093/nar/gkaf018.

MethylKit

Morgan, M., & Huber, W. (2020). methylKit: a package for the analysis of DNA methylation data in R. Bioinformatics, 36(1), 214-216. https://doi.org/10.1093/bioinformatics/btz262

ggplot2

Wickham H (2016). ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag New York. ISBN 978-3-319-24277-4, https://ggplot2.tidyverse.org.

Gene Ontology

Xu S, Hu E, Cai Y, Xie Z, Luo X, Zhan L, Tang W, Wang Q, Liu B, Wang R, Xie W, Wu T, Xie L, Yu G (2024). “Using clusterProfiler to characterize multiomics data.” Nature Protocols, 19(11), 3292-3320. doi:10.1038/s41596-024-01020-z, https://www.nature.com/articles/s41596-024-01020-z.

Walter, Wencke, Fátima Sánchez-Cabo, and Mercedes Ricote. "GOplot: an R package for visually combining expression data with functional analysis." Bioinformatics (2015): btv300.

Software packaging/containerisation tools

Anaconda

Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web.

Bioconda

Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506.

BioContainers

da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671.

Docker

Merkel, D. (2014). Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014(239), 2. doi: 10.5555/2600239.2600241.

Nextflow

Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311.

R and Bioconductor

R. Ihaka and R. Gentleman (1996), R: A Language for Data Analysis and Graphics, Journal of Computational and Graphical Statistics, Vol 5, No 3, 299-314.

Huber, W., Carey, V., Gentleman, R. et al. Orchestrating high-throughput genomic analysis with Bioconductor. Nat Methods 12, 115–121 (2015). https://doi.org/10.1038/nmeth.3252.

Singularity

Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675.